Extracts of Jamun seeds inhibited the growth of human (Hep‑2) cancer cells
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Date
2023-04-07
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Journal of Cancer Research and Therapeutics | Published by Wolters Kluwer - Medknow
Abstract
Introduction: In the last century, the human laryngeal epithelioma has become a life‑threatening disease leading to a high rate of
mortality worldwide. The current investigation is focusing on the antiproliferative effect of Eugenia jambolana seed extracts against
Hep‑2 cancer cells.
Methods: The active compounds from the seeds of E. jambolana were extracted by the decoction extraction method using acetone,
ethanol, and methanol. The filtrates from the different solvents were subjected to liquid‑liquid separation before drying by a rotary
evaporator. In various doses, the crude extracts and carcinoma were subjected to a methylthiazolyl diphenyl tetrazolium bromide
assay. Cell viability was determined under ultraviolet visualization at an absorbance of 540 nm. The data of the viable cells were
subjected to analysis of variance at P ≤ .01.
Results: Crude compounds of E. jambolana seeds extracted by acetone, methanol, and methanol extract had an anticarcinoma
effect. Among the extracts, methanol extract possessed a recommendable anti‑carcinoma effect compared to acetone and ethanol
crude extracts. At a concentration of 125 µg/mL, the crude extracts of methanol, acetone, and ethanol destroyed 49.57, 35.01,
and 27.67 carcinomas, respectively. The concentration of 31.25 µg/mL of acetone extract and 125 µg/mL of ethanolic extract
affected 28.11 and 27.67 carcinomas, respectively.
Conclusions: E. jambolana seeds possess anticarcinoma potency and thus can be administered in the reduction of proliferative
carcinoma. The study recommended further studies which will involve the elution of pure compounds from the methanol extract of
E. jambolana that possess antitumour and antiproliferative activity against Hep‑2 cell lines.
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Keywords
Anti‑carcinoma; Eugenia jambolana; Human laryngeal epithelioma type 2 (Hep‑2) cells